ʻO ka kānana Fluorescence kahi mea nui i hoʻohana ʻia i nā mea hana biomedical a me ka ʻepekema ola.ʻO kāna hana nui, ʻo ia ke hoʻokaʻawale a koho i ke kikoʻī o ka hawewe lōʻihi mai ka māmā excitation a me ka fluorescence hoʻokuʻu o ka mea i loko o ka biomedical fluorescence inspection and analysis system.Hōʻike pinepine ʻia nā kānana fluorescence e kahi hohonu ʻoki ʻoki a me ka autofluorescence haʻahaʻa.ʻO ka mea maʻamau, hiki ke hoʻopili ʻia nā kānana he nui e hana i kahi kānana fluorescence, a he kiʻekiʻe ke kumu kūʻai.
Hoʻohana ʻia ka kānana Fluorescence i ka mea hana PCR fluorescence quantitative maoli.ka mea i hoʻohana nui ʻia i ka molecular biology a me ka ʻike palekana meaʻai a me ka nānā ʻana i ka maʻi maʻi olakino a me nā ʻoihana ʻē aʻe.
Hoʻohana ʻia ka kānana Fluorescence no ka ʻike ʻana i nā ʻano like ʻole a me nā mea ʻimi, no ka laʻana:
FAM/SYBR Green/ Green/ HEX/TET/Cy3/JOE/ ROX/Cy3.5/Texas Red,Cy5/LC Red640,Cy5.5 etc.
Kaʻina hana | (Ka uhi paʻa IAD) | ||
Loʻihi nalu | Ex(nm) | Em(nm) | Kea |
| 470-30 | 525-20 | >6 |
| 523-20 | 564-20 | >6 |
| 543-20 | 584-20 | >6 |
| 571-20 | 612-20 | >6 |
| 628-35 | 692-45 | >6 |
Kāohi ʻana | OD>6@200~900nm a i ʻole @200~1200nm | ||
Piʻi(nm) | 50 %~ OD5 <10nm | ||
Kea | OD>6 | ||
Nui (mm) | Φ4mm, Φ12mm,Φ12.7mm,Φ25.4mm etc. |